Improving one-step scarless genome editing in Drosophila melanogaster by combining ovo(D) co-CRISPR selection with sgRNA target site masking

The precise and rapid construction of alleles through CRISPR/Cas9-mediated genome engineering renders Drosophila melanogaster a powerful animal system for molecular structure–function analyses and human disease models. Application of the ovo(D) co-selection method offers expedited generation and enr...

Descripción completa

Detalles Bibliográficos
Autores principales: Götze, Katharina J, Mrestani, Achmed, Beckmann, Paula, Krohn, Knut, Le Duc, Diana, Velluva, Akhil, Böhme, Mathias A, Heckmann, Manfred, Abou Jamra, Rami, Lemke, Johannes R, Bläker, Hendrik, Scholz, Nicole, Ljaschenko, Dmitrij, Langenhan, Tobias
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Methods Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8789338/
https://www.ncbi.nlm.nih.gov/pubmed/35087953
http://dx.doi.org/10.1093/biomethods/bpac003

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8789338/
https://www.ncbi.nlm.nih.gov/pubmed/35087953
http://dx.doi.org/10.1093/biomethods/bpac003

Ejemplares similares