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Species-resolved sequencing of low-biomass or degraded microbiomes using 2bRAD-M

Microbiome samples with low microbial biomass or severe DNA degradation remain challenging for amplicon-based or whole-metagenome sequencing approaches. Here, we introduce 2bRAD-M, a highly reduced and cost-effective strategy which only sequences ~ 1% of metagenome and can simultaneously produce spe...

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Detalles Bibliográficos
Autores principales: Sun, Zheng, Huang, Shi, Zhu, Pengfei, Tzehau, Lam, Zhao, Helen, Lv, Jia, Zhang, Rongchao, Zhou, Lisha, Niu, Qianya, Wang, Xiuping, Zhang, Meng, Jing, Gongchao, Bao, Zhenmin, Liu, Jiquan, Wang, Shi, Xu, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8789378/
https://www.ncbi.nlm.nih.gov/pubmed/35078506
http://dx.doi.org/10.1186/s13059-021-02576-9