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Species-resolved sequencing of low-biomass or degraded microbiomes using 2bRAD-M
Microbiome samples with low microbial biomass or severe DNA degradation remain challenging for amplicon-based or whole-metagenome sequencing approaches. Here, we introduce 2bRAD-M, a highly reduced and cost-effective strategy which only sequences ~ 1% of metagenome and can simultaneously produce spe...
Autores principales: | Sun, Zheng, Huang, Shi, Zhu, Pengfei, Tzehau, Lam, Zhao, Helen, Lv, Jia, Zhang, Rongchao, Zhou, Lisha, Niu, Qianya, Wang, Xiuping, Zhang, Meng, Jing, Gongchao, Bao, Zhenmin, Liu, Jiquan, Wang, Shi, Xu, Jian |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8789378/ https://www.ncbi.nlm.nih.gov/pubmed/35078506 http://dx.doi.org/10.1186/s13059-021-02576-9 |
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