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Rif2 protects Rap1-depleted telomeres from MRX-mediated degradation in Saccharomyces cerevisiae
Rap1 is the main protein that binds double-stranded telomeric DNA in Saccharomyces cerevisiae. Examination of the telomere functions of Rap1 is complicated by the fact that it also acts as a transcriptional regulator of hundreds of genes and is encoded by an essential gene. In this study, we disrupt...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8791636/ https://www.ncbi.nlm.nih.gov/pubmed/35044907 http://dx.doi.org/10.7554/eLife.74090 |
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author | Rosas Bringas, Fernando Rodrigo Stinus, Sonia de Zoeten, Pien Cohn, Marita Chang, Michael |
author_facet | Rosas Bringas, Fernando Rodrigo Stinus, Sonia de Zoeten, Pien Cohn, Marita Chang, Michael |
author_sort | Rosas Bringas, Fernando Rodrigo |
collection | PubMed |
description | Rap1 is the main protein that binds double-stranded telomeric DNA in Saccharomyces cerevisiae. Examination of the telomere functions of Rap1 is complicated by the fact that it also acts as a transcriptional regulator of hundreds of genes and is encoded by an essential gene. In this study, we disrupt Rap1 telomere association by expressing a mutant telomerase RNA subunit (tlc1-tm) that introduces mutant telomeric repeats. tlc1-tm cells grow similar to wild-type cells, although depletion of Rap1 at telomeres causes defects in telomere length regulation and telomere capping. Rif2 is a protein normally recruited to telomeres by Rap1, but we show that Rif2 can still associate with Rap1-depleted tlc1-tm telomeres, and that this association is required to inhibit telomere degradation by the MRX complex. Rif2 and the Ku complex work in parallel to prevent tlc1-tm telomere degradation; tlc1-tm cells lacking Rif2 and the Ku complex are inviable. The partially redundant mechanisms may explain the rapid evolution of telomere components in budding yeast species. |
format | Online Article Text |
id | pubmed-8791636 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-87916362022-01-27 Rif2 protects Rap1-depleted telomeres from MRX-mediated degradation in Saccharomyces cerevisiae Rosas Bringas, Fernando Rodrigo Stinus, Sonia de Zoeten, Pien Cohn, Marita Chang, Michael eLife Chromosomes and Gene Expression Rap1 is the main protein that binds double-stranded telomeric DNA in Saccharomyces cerevisiae. Examination of the telomere functions of Rap1 is complicated by the fact that it also acts as a transcriptional regulator of hundreds of genes and is encoded by an essential gene. In this study, we disrupt Rap1 telomere association by expressing a mutant telomerase RNA subunit (tlc1-tm) that introduces mutant telomeric repeats. tlc1-tm cells grow similar to wild-type cells, although depletion of Rap1 at telomeres causes defects in telomere length regulation and telomere capping. Rif2 is a protein normally recruited to telomeres by Rap1, but we show that Rif2 can still associate with Rap1-depleted tlc1-tm telomeres, and that this association is required to inhibit telomere degradation by the MRX complex. Rif2 and the Ku complex work in parallel to prevent tlc1-tm telomere degradation; tlc1-tm cells lacking Rif2 and the Ku complex are inviable. The partially redundant mechanisms may explain the rapid evolution of telomere components in budding yeast species. eLife Sciences Publications, Ltd 2022-01-19 /pmc/articles/PMC8791636/ /pubmed/35044907 http://dx.doi.org/10.7554/eLife.74090 Text en © 2022, Rosas Bringas et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Chromosomes and Gene Expression Rosas Bringas, Fernando Rodrigo Stinus, Sonia de Zoeten, Pien Cohn, Marita Chang, Michael Rif2 protects Rap1-depleted telomeres from MRX-mediated degradation in Saccharomyces cerevisiae |
title | Rif2 protects Rap1-depleted telomeres from MRX-mediated degradation in Saccharomyces cerevisiae |
title_full | Rif2 protects Rap1-depleted telomeres from MRX-mediated degradation in Saccharomyces cerevisiae |
title_fullStr | Rif2 protects Rap1-depleted telomeres from MRX-mediated degradation in Saccharomyces cerevisiae |
title_full_unstemmed | Rif2 protects Rap1-depleted telomeres from MRX-mediated degradation in Saccharomyces cerevisiae |
title_short | Rif2 protects Rap1-depleted telomeres from MRX-mediated degradation in Saccharomyces cerevisiae |
title_sort | rif2 protects rap1-depleted telomeres from mrx-mediated degradation in saccharomyces cerevisiae |
topic | Chromosomes and Gene Expression |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8791636/ https://www.ncbi.nlm.nih.gov/pubmed/35044907 http://dx.doi.org/10.7554/eLife.74090 |
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