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A generic protocol for the affinity-purification of native macromolecular complexes from poxvirus-infected cells
The functional and structural characterization of macromolecular complexes requires protocols for their native isolation. Here, we describe a protocol for this task based on the recombinant poxvirus Vaccinia expressing tagged proteins of interest in infected cells. Tagged proteins and their interact...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8792428/ https://www.ncbi.nlm.nih.gov/pubmed/35118428 http://dx.doi.org/10.1016/j.xpro.2021.101116 |