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A generic protocol for the affinity-purification of native macromolecular complexes from poxvirus-infected cells

The functional and structural characterization of macromolecular complexes requires protocols for their native isolation. Here, we describe a protocol for this task based on the recombinant poxvirus Vaccinia expressing tagged proteins of interest in infected cells. Tagged proteins and their interact...

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Detalles Bibliográficos
Autores principales: Bartuli, Julia, Lorenzi, Isotta, Backes, Simone, Grimm, Clemens, Fischer, Utz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8792428/
https://www.ncbi.nlm.nih.gov/pubmed/35118428
http://dx.doi.org/10.1016/j.xpro.2021.101116

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