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A 2D and 3D cell culture protocol to study O-GlcNAc in sphingosine-1-phosphate mediated fibroblast contraction

O-linked β-N-acetylglucosamine (O-GlcNAc) is an abundant posttranslational modification involved in a wide range of signaling pathways, but its specific role in regulating biological processes remains unclear. This protocol describes approaches to understand O-GlcNAc’s role in fibroblast contraction...

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Detalles Bibliográficos
Autores principales: Morales, Murielle M., Pedowitz, Nichole J., Pratt, Matthew R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8792430/
https://www.ncbi.nlm.nih.gov/pubmed/35118425
http://dx.doi.org/10.1016/j.xpro.2021.101113