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A 2D and 3D cell culture protocol to study O-GlcNAc in sphingosine-1-phosphate mediated fibroblast contraction
O-linked β-N-acetylglucosamine (O-GlcNAc) is an abundant posttranslational modification involved in a wide range of signaling pathways, but its specific role in regulating biological processes remains unclear. This protocol describes approaches to understand O-GlcNAc’s role in fibroblast contraction...
Autores principales: | Morales, Murielle M., Pedowitz, Nichole J., Pratt, Matthew R. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8792430/ https://www.ncbi.nlm.nih.gov/pubmed/35118425 http://dx.doi.org/10.1016/j.xpro.2021.101113 |
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