Protocol for whole-mount X-gal staining combined with tissue clearing in embryo and adult mouse using CUBIC

Here we describe an optimized protocol for X-gal staining of tissue clearing embryo and adult mouse using CUBIC. The activity of LacZ knock-in reflecting endogenous expression of genes of interest in the whole body can be visualized by X-gal staining. This protocol is suitable for examining the deve...

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Detalles Bibliográficos
Autores principales: Watanabe-Takano, Haruko, Fukumoto, Moe, Fukuhara, Shigetomo, Mochizuki, Naoki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8792446/
https://www.ncbi.nlm.nih.gov/pubmed/35118431
http://dx.doi.org/10.1016/j.xpro.2022.101127

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8792446/
https://www.ncbi.nlm.nih.gov/pubmed/35118431
http://dx.doi.org/10.1016/j.xpro.2022.101127

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