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Construction of Aerobic/Anaerobic-Substrate-Induced Gene Expression Procedure for Exploration of Metagenomes From Subseafloor Sediments

Substrate-induced gene expression (SIGEX) is a high-throughput promoter-trap method. It is a function-based metagenomic screening tool that relies on transcriptional activation of a reporter gene green fluorescence protein (gfp) by a metagenomic DNA library upon induction with a substrate. However,...

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Detalles Bibliográficos
Autores principales: Wakamatsu, Taisuke, Mizobuchi, Saki, Mori, Fumiaki, Futagami, Taiki, Terada, Takeshi, Morono, Yuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8793675/
https://www.ncbi.nlm.nih.gov/pubmed/35095779
http://dx.doi.org/10.3389/fmicb.2021.726024