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Construction of Aerobic/Anaerobic-Substrate-Induced Gene Expression Procedure for Exploration of Metagenomes From Subseafloor Sediments
Substrate-induced gene expression (SIGEX) is a high-throughput promoter-trap method. It is a function-based metagenomic screening tool that relies on transcriptional activation of a reporter gene green fluorescence protein (gfp) by a metagenomic DNA library upon induction with a substrate. However,...
Autores principales: | Wakamatsu, Taisuke, Mizobuchi, Saki, Mori, Fumiaki, Futagami, Taiki, Terada, Takeshi, Morono, Yuki |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8793675/ https://www.ncbi.nlm.nih.gov/pubmed/35095779 http://dx.doi.org/10.3389/fmicb.2021.726024 |
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