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A compensatory link between cleavage/polyadenylation and mRNA turnover regulates steady-state mRNA levels in yeast

Cells have compensatory mechanisms to coordinate the rates of major biological processes, thereby permitting growth in a wide variety of conditions. Here, we uncover a compensatory link between cleavage/polyadenylation in the nucleus and messenger RNA (mRNA) turnover in the cytoplasm. On a global ba...

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Autores principales: Moqtaderi, Zarmik, Geisberg, Joseph V., Struhl, Kevin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8794773/
https://www.ncbi.nlm.nih.gov/pubmed/35058367
http://dx.doi.org/10.1073/pnas.2121488119
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author Moqtaderi, Zarmik
Geisberg, Joseph V.
Struhl, Kevin
author_facet Moqtaderi, Zarmik
Geisberg, Joseph V.
Struhl, Kevin
author_sort Moqtaderi, Zarmik
collection PubMed
description Cells have compensatory mechanisms to coordinate the rates of major biological processes, thereby permitting growth in a wide variety of conditions. Here, we uncover a compensatory link between cleavage/polyadenylation in the nucleus and messenger RNA (mRNA) turnover in the cytoplasm. On a global basis, same-gene 3′ mRNA isoforms with twofold or greater differences in half-lives have steady-state mRNA levels that differ by significantly less than a factor of 2. In addition, increased efficiency of cleavage/polyadenylation at a specific site is associated with reduced stability of the corresponding 3′ mRNA isoform. This inverse relationship between cleavage/polyadenylation and mRNA isoform half-life reduces the variability in the steady-state levels of mRNA isoforms, and it occurs in all four growth conditions tested. These observations suggest that during cleavage/polyadenylation in the nucleus, mRNA isoforms are marked in a manner that persists upon translocation to the cytoplasm and affects the activity of mRNA degradation machinery, thus influencing mRNA stability.
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spelling pubmed-87947732022-07-20 A compensatory link between cleavage/polyadenylation and mRNA turnover regulates steady-state mRNA levels in yeast Moqtaderi, Zarmik Geisberg, Joseph V. Struhl, Kevin Proc Natl Acad Sci U S A Biological Sciences Cells have compensatory mechanisms to coordinate the rates of major biological processes, thereby permitting growth in a wide variety of conditions. Here, we uncover a compensatory link between cleavage/polyadenylation in the nucleus and messenger RNA (mRNA) turnover in the cytoplasm. On a global basis, same-gene 3′ mRNA isoforms with twofold or greater differences in half-lives have steady-state mRNA levels that differ by significantly less than a factor of 2. In addition, increased efficiency of cleavage/polyadenylation at a specific site is associated with reduced stability of the corresponding 3′ mRNA isoform. This inverse relationship between cleavage/polyadenylation and mRNA isoform half-life reduces the variability in the steady-state levels of mRNA isoforms, and it occurs in all four growth conditions tested. These observations suggest that during cleavage/polyadenylation in the nucleus, mRNA isoforms are marked in a manner that persists upon translocation to the cytoplasm and affects the activity of mRNA degradation machinery, thus influencing mRNA stability. National Academy of Sciences 2022-01-20 2022-01-25 /pmc/articles/PMC8794773/ /pubmed/35058367 http://dx.doi.org/10.1073/pnas.2121488119 Text en Copyright © 2022 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Biological Sciences
Moqtaderi, Zarmik
Geisberg, Joseph V.
Struhl, Kevin
A compensatory link between cleavage/polyadenylation and mRNA turnover regulates steady-state mRNA levels in yeast
title A compensatory link between cleavage/polyadenylation and mRNA turnover regulates steady-state mRNA levels in yeast
title_full A compensatory link between cleavage/polyadenylation and mRNA turnover regulates steady-state mRNA levels in yeast
title_fullStr A compensatory link between cleavage/polyadenylation and mRNA turnover regulates steady-state mRNA levels in yeast
title_full_unstemmed A compensatory link between cleavage/polyadenylation and mRNA turnover regulates steady-state mRNA levels in yeast
title_short A compensatory link between cleavage/polyadenylation and mRNA turnover regulates steady-state mRNA levels in yeast
title_sort compensatory link between cleavage/polyadenylation and mrna turnover regulates steady-state mrna levels in yeast
topic Biological Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8794773/
https://www.ncbi.nlm.nih.gov/pubmed/35058367
http://dx.doi.org/10.1073/pnas.2121488119
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