T7-lac promoter vectors spontaneous derepression caused by plant-derived growth media may lead to serious expression problems: a systematic evaluation

BACKGROUND: The widespread usage of protein expression systems in Escherichia coli (E. coli) is a workhorse of molecular biology research that has practical applications in biotechnology industry, including the production of pharmaceutical drugs. Various factors can strongly affect the successful co...

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Detalles Bibliográficos
Autores principales: Krefft, Daria, Prusinowski, Maciej, Maciszka, Paulina, Skokowska, Aleksandra, Zebrowska, Joanna, Skowron, Piotr M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8796431/
https://www.ncbi.nlm.nih.gov/pubmed/35090462
http://dx.doi.org/10.1186/s12934-022-01740-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8796431/
https://www.ncbi.nlm.nih.gov/pubmed/35090462
http://dx.doi.org/10.1186/s12934-022-01740-5

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