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IL-35 inhibits CD8(+) T cells activity by suppressing expression of costimulatory molecule CD28 and Th1 cytokine production
BACKGROUND: Interleukin-35 (IL-35), a novel immune-suppressing cytokine, can promote tumor angiogenesis and inhibits anti-tumor cytotoxic lymphocyte response. Here, we aimed to investigate the potential mechanism of the effects of IL-35 on anti-tumor cytotoxic lymphocyte. METHODS: Dendritic cells (D...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AME Publishing Company
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8797787/ https://www.ncbi.nlm.nih.gov/pubmed/35116874 http://dx.doi.org/10.21037/tcr.2019.07.30 |
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author | Jiang, Hua Zhang, Ting Yan, Mao-Xiao Wu, Wei |
author_facet | Jiang, Hua Zhang, Ting Yan, Mao-Xiao Wu, Wei |
author_sort | Jiang, Hua |
collection | PubMed |
description | BACKGROUND: Interleukin-35 (IL-35), a novel immune-suppressing cytokine, can promote tumor angiogenesis and inhibits anti-tumor cytotoxic lymphocyte response. Here, we aimed to investigate the potential mechanism of the effects of IL-35 on anti-tumor cytotoxic lymphocyte. METHODS: Dendritic cells (DCs) were used to induce anti-tumor cytotoxic lymphocyte. Flow cytometry, carboxyfluorescein succinimidyl ester staining, ELISA assay and western blotting were used to analyze the effect of IL-35 on anti-tumor cytotoxic lymphocyte. RESULTS: We observed that IL-35 inhibited the expression of costimulatory molecule CD28 on CD8(+) T cell surface and Th1 cytokine production. However, IL-35 did not inhibit anti-tumor cytotoxic lymphocyte proliferation nor enhance the expression of apoptosis-related proteins of anti-tumor cytotoxic lymphocyte. Moreover, IL-35 did not repress the expression of Fas ligand (FasL) on cytotoxic lymphocyte surface. CONCLUSIONS: Our findings revealed that IL-35 can inhibit CD8(+) T cells activity by suppressing the expression of costimulatory molecule CD28 and Th1 cytokine production. |
format | Online Article Text |
id | pubmed-8797787 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | AME Publishing Company |
record_format | MEDLINE/PubMed |
spelling | pubmed-87977872022-02-02 IL-35 inhibits CD8(+) T cells activity by suppressing expression of costimulatory molecule CD28 and Th1 cytokine production Jiang, Hua Zhang, Ting Yan, Mao-Xiao Wu, Wei Transl Cancer Res Original Article BACKGROUND: Interleukin-35 (IL-35), a novel immune-suppressing cytokine, can promote tumor angiogenesis and inhibits anti-tumor cytotoxic lymphocyte response. Here, we aimed to investigate the potential mechanism of the effects of IL-35 on anti-tumor cytotoxic lymphocyte. METHODS: Dendritic cells (DCs) were used to induce anti-tumor cytotoxic lymphocyte. Flow cytometry, carboxyfluorescein succinimidyl ester staining, ELISA assay and western blotting were used to analyze the effect of IL-35 on anti-tumor cytotoxic lymphocyte. RESULTS: We observed that IL-35 inhibited the expression of costimulatory molecule CD28 on CD8(+) T cell surface and Th1 cytokine production. However, IL-35 did not inhibit anti-tumor cytotoxic lymphocyte proliferation nor enhance the expression of apoptosis-related proteins of anti-tumor cytotoxic lymphocyte. Moreover, IL-35 did not repress the expression of Fas ligand (FasL) on cytotoxic lymphocyte surface. CONCLUSIONS: Our findings revealed that IL-35 can inhibit CD8(+) T cells activity by suppressing the expression of costimulatory molecule CD28 and Th1 cytokine production. AME Publishing Company 2019-08 /pmc/articles/PMC8797787/ /pubmed/35116874 http://dx.doi.org/10.21037/tcr.2019.07.30 Text en 2019 Translational Cancer Research. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0/. |
spellingShingle | Original Article Jiang, Hua Zhang, Ting Yan, Mao-Xiao Wu, Wei IL-35 inhibits CD8(+) T cells activity by suppressing expression of costimulatory molecule CD28 and Th1 cytokine production |
title | IL-35 inhibits CD8(+) T cells activity by suppressing expression of costimulatory molecule CD28 and Th1 cytokine production |
title_full | IL-35 inhibits CD8(+) T cells activity by suppressing expression of costimulatory molecule CD28 and Th1 cytokine production |
title_fullStr | IL-35 inhibits CD8(+) T cells activity by suppressing expression of costimulatory molecule CD28 and Th1 cytokine production |
title_full_unstemmed | IL-35 inhibits CD8(+) T cells activity by suppressing expression of costimulatory molecule CD28 and Th1 cytokine production |
title_short | IL-35 inhibits CD8(+) T cells activity by suppressing expression of costimulatory molecule CD28 and Th1 cytokine production |
title_sort | il-35 inhibits cd8(+) t cells activity by suppressing expression of costimulatory molecule cd28 and th1 cytokine production |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8797787/ https://www.ncbi.nlm.nih.gov/pubmed/35116874 http://dx.doi.org/10.21037/tcr.2019.07.30 |
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