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IL-35 inhibits CD8(+) T cells activity by suppressing expression of costimulatory molecule CD28 and Th1 cytokine production

BACKGROUND: Interleukin-35 (IL-35), a novel immune-suppressing cytokine, can promote tumor angiogenesis and inhibits anti-tumor cytotoxic lymphocyte response. Here, we aimed to investigate the potential mechanism of the effects of IL-35 on anti-tumor cytotoxic lymphocyte. METHODS: Dendritic cells (D...

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Detalles Bibliográficos
Autores principales: Jiang, Hua, Zhang, Ting, Yan, Mao-Xiao, Wu, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AME Publishing Company 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8797787/
https://www.ncbi.nlm.nih.gov/pubmed/35116874
http://dx.doi.org/10.21037/tcr.2019.07.30
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author Jiang, Hua
Zhang, Ting
Yan, Mao-Xiao
Wu, Wei
author_facet Jiang, Hua
Zhang, Ting
Yan, Mao-Xiao
Wu, Wei
author_sort Jiang, Hua
collection PubMed
description BACKGROUND: Interleukin-35 (IL-35), a novel immune-suppressing cytokine, can promote tumor angiogenesis and inhibits anti-tumor cytotoxic lymphocyte response. Here, we aimed to investigate the potential mechanism of the effects of IL-35 on anti-tumor cytotoxic lymphocyte. METHODS: Dendritic cells (DCs) were used to induce anti-tumor cytotoxic lymphocyte. Flow cytometry, carboxyfluorescein succinimidyl ester staining, ELISA assay and western blotting were used to analyze the effect of IL-35 on anti-tumor cytotoxic lymphocyte. RESULTS: We observed that IL-35 inhibited the expression of costimulatory molecule CD28 on CD8(+) T cell surface and Th1 cytokine production. However, IL-35 did not inhibit anti-tumor cytotoxic lymphocyte proliferation nor enhance the expression of apoptosis-related proteins of anti-tumor cytotoxic lymphocyte. Moreover, IL-35 did not repress the expression of Fas ligand (FasL) on cytotoxic lymphocyte surface. CONCLUSIONS: Our findings revealed that IL-35 can inhibit CD8(+) T cells activity by suppressing the expression of costimulatory molecule CD28 and Th1 cytokine production.
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spelling pubmed-87977872022-02-02 IL-35 inhibits CD8(+) T cells activity by suppressing expression of costimulatory molecule CD28 and Th1 cytokine production Jiang, Hua Zhang, Ting Yan, Mao-Xiao Wu, Wei Transl Cancer Res Original Article BACKGROUND: Interleukin-35 (IL-35), a novel immune-suppressing cytokine, can promote tumor angiogenesis and inhibits anti-tumor cytotoxic lymphocyte response. Here, we aimed to investigate the potential mechanism of the effects of IL-35 on anti-tumor cytotoxic lymphocyte. METHODS: Dendritic cells (DCs) were used to induce anti-tumor cytotoxic lymphocyte. Flow cytometry, carboxyfluorescein succinimidyl ester staining, ELISA assay and western blotting were used to analyze the effect of IL-35 on anti-tumor cytotoxic lymphocyte. RESULTS: We observed that IL-35 inhibited the expression of costimulatory molecule CD28 on CD8(+) T cell surface and Th1 cytokine production. However, IL-35 did not inhibit anti-tumor cytotoxic lymphocyte proliferation nor enhance the expression of apoptosis-related proteins of anti-tumor cytotoxic lymphocyte. Moreover, IL-35 did not repress the expression of Fas ligand (FasL) on cytotoxic lymphocyte surface. CONCLUSIONS: Our findings revealed that IL-35 can inhibit CD8(+) T cells activity by suppressing the expression of costimulatory molecule CD28 and Th1 cytokine production. AME Publishing Company 2019-08 /pmc/articles/PMC8797787/ /pubmed/35116874 http://dx.doi.org/10.21037/tcr.2019.07.30 Text en 2019 Translational Cancer Research. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0/.
spellingShingle Original Article
Jiang, Hua
Zhang, Ting
Yan, Mao-Xiao
Wu, Wei
IL-35 inhibits CD8(+) T cells activity by suppressing expression of costimulatory molecule CD28 and Th1 cytokine production
title IL-35 inhibits CD8(+) T cells activity by suppressing expression of costimulatory molecule CD28 and Th1 cytokine production
title_full IL-35 inhibits CD8(+) T cells activity by suppressing expression of costimulatory molecule CD28 and Th1 cytokine production
title_fullStr IL-35 inhibits CD8(+) T cells activity by suppressing expression of costimulatory molecule CD28 and Th1 cytokine production
title_full_unstemmed IL-35 inhibits CD8(+) T cells activity by suppressing expression of costimulatory molecule CD28 and Th1 cytokine production
title_short IL-35 inhibits CD8(+) T cells activity by suppressing expression of costimulatory molecule CD28 and Th1 cytokine production
title_sort il-35 inhibits cd8(+) t cells activity by suppressing expression of costimulatory molecule cd28 and th1 cytokine production
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8797787/
https://www.ncbi.nlm.nih.gov/pubmed/35116874
http://dx.doi.org/10.21037/tcr.2019.07.30
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