Protocols for marker-free gene knock-out and knock-down in Kluyveromyces marxianus using CRISPR/Cas9

There is increased interest in strain engineering in the food and industrial yeast Kluyveromyces marxianus and a number of CRISPR/Cas9 systems have been described and used by different groups. The methods that we developed allow for very rapid and efficient inactivation of target genes using the end...

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Detalles Bibliográficos
Autores principales: Rajkumar, Arun S, Morrissey, John P
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8800938/
https://www.ncbi.nlm.nih.gov/pubmed/34928332
http://dx.doi.org/10.1093/femsyr/foab067

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8800938/
https://www.ncbi.nlm.nih.gov/pubmed/34928332
http://dx.doi.org/10.1093/femsyr/foab067

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