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Detecting microRNA-mediated gene regulatory effects in murine neuronal subpopulations
microRNAs (miRNAs) have unique gene regulatory effects in different neuronal subpopulations. Here, we describe a protocol to identify neuronal subtype-specific effects of a miRNA in murine motor neuron subpopulations. We detail the preparation of primary mouse spinal tissue for single cell RNA seque...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8801384/ https://www.ncbi.nlm.nih.gov/pubmed/35146446 http://dx.doi.org/10.1016/j.xpro.2022.101130 |