Detecting microRNA-mediated gene regulatory effects in murine neuronal subpopulations

microRNAs (miRNAs) have unique gene regulatory effects in different neuronal subpopulations. Here, we describe a protocol to identify neuronal subtype-specific effects of a miRNA in murine motor neuron subpopulations. We detail the preparation of primary mouse spinal tissue for single cell RNA seque...

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Detalles Bibliográficos
Autores principales: Amin, Neal D., Senturk, Gokhan, Hayashi, Marito, Driscoll, Shawn P., Pfaff, Samuel L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8801384/
https://www.ncbi.nlm.nih.gov/pubmed/35146446
http://dx.doi.org/10.1016/j.xpro.2022.101130

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