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Enhancement of proliferation of human umbilical cord blood–derived CD34(+) hematopoietic stem cells by a combination of hyper-interleukin-6 and small molecules
Umbilical cord blood (UCB) is an alternative source of allogeneic hematopoietic stem cells (HSCs) for transplantation to treat various hematological disorders. The major limitation to the use of UCB-derived HSCs (UCB–HSCs) in transplantation, however, is the low numbers of HSCs in a unit of cord blo...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8801758/ https://www.ncbi.nlm.nih.gov/pubmed/35146134 http://dx.doi.org/10.1016/j.bbrep.2022.101214 |
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author | Park, Yo Seph Lee, Yukyeong Choi, Na Young Hwang, Han Sung Rose-John, Stefan Zenke, Martin Ko, Kinarm |
author_facet | Park, Yo Seph Lee, Yukyeong Choi, Na Young Hwang, Han Sung Rose-John, Stefan Zenke, Martin Ko, Kinarm |
author_sort | Park, Yo Seph |
collection | PubMed |
description | Umbilical cord blood (UCB) is an alternative source of allogeneic hematopoietic stem cells (HSCs) for transplantation to treat various hematological disorders. The major limitation to the use of UCB-derived HSCs (UCB–HSCs) in transplantation, however, is the low numbers of HSCs in a unit of cord blood. To overcome this limitation, various cytokines or small molecules have been used to expand UCB-HSCs ex vivo. In this study, we investigated a synergistic effect of the combination of HIL-6, SR1, and UM171 on UCB-HSC culture and found that this combination resulted in the highest number of CD34(+) cells. These results suggest that the combination of SR1, UM171 and HIL-6 exerts a synergistic effect in the proliferation of HSCs from UCB and thus, SR1, UM171 and HIL-6 is the most suitable combination for obtaining HSCs from UCB for clinical transplantation. |
format | Online Article Text |
id | pubmed-8801758 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-88017582022-02-09 Enhancement of proliferation of human umbilical cord blood–derived CD34(+) hematopoietic stem cells by a combination of hyper-interleukin-6 and small molecules Park, Yo Seph Lee, Yukyeong Choi, Na Young Hwang, Han Sung Rose-John, Stefan Zenke, Martin Ko, Kinarm Biochem Biophys Rep Short Communication Umbilical cord blood (UCB) is an alternative source of allogeneic hematopoietic stem cells (HSCs) for transplantation to treat various hematological disorders. The major limitation to the use of UCB-derived HSCs (UCB–HSCs) in transplantation, however, is the low numbers of HSCs in a unit of cord blood. To overcome this limitation, various cytokines or small molecules have been used to expand UCB-HSCs ex vivo. In this study, we investigated a synergistic effect of the combination of HIL-6, SR1, and UM171 on UCB-HSC culture and found that this combination resulted in the highest number of CD34(+) cells. These results suggest that the combination of SR1, UM171 and HIL-6 exerts a synergistic effect in the proliferation of HSCs from UCB and thus, SR1, UM171 and HIL-6 is the most suitable combination for obtaining HSCs from UCB for clinical transplantation. Elsevier 2022-01-27 /pmc/articles/PMC8801758/ /pubmed/35146134 http://dx.doi.org/10.1016/j.bbrep.2022.101214 Text en © 2022 The Authors. Published by Elsevier B.V. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Short Communication Park, Yo Seph Lee, Yukyeong Choi, Na Young Hwang, Han Sung Rose-John, Stefan Zenke, Martin Ko, Kinarm Enhancement of proliferation of human umbilical cord blood–derived CD34(+) hematopoietic stem cells by a combination of hyper-interleukin-6 and small molecules |
title | Enhancement of proliferation of human umbilical cord blood–derived CD34(+) hematopoietic stem cells by a combination of hyper-interleukin-6 and small molecules |
title_full | Enhancement of proliferation of human umbilical cord blood–derived CD34(+) hematopoietic stem cells by a combination of hyper-interleukin-6 and small molecules |
title_fullStr | Enhancement of proliferation of human umbilical cord blood–derived CD34(+) hematopoietic stem cells by a combination of hyper-interleukin-6 and small molecules |
title_full_unstemmed | Enhancement of proliferation of human umbilical cord blood–derived CD34(+) hematopoietic stem cells by a combination of hyper-interleukin-6 and small molecules |
title_short | Enhancement of proliferation of human umbilical cord blood–derived CD34(+) hematopoietic stem cells by a combination of hyper-interleukin-6 and small molecules |
title_sort | enhancement of proliferation of human umbilical cord blood–derived cd34(+) hematopoietic stem cells by a combination of hyper-interleukin-6 and small molecules |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8801758/ https://www.ncbi.nlm.nih.gov/pubmed/35146134 http://dx.doi.org/10.1016/j.bbrep.2022.101214 |
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