Evaluation of a fit-for-purpose assay to monitor antigen-specific functional CD4+ T-cell subpopulations in rheumatoid arthritis using flow cytometry–based peptide-MHC class-II tetramer staining

Antigen-specific T cells can serve as a response biomarker in non-clinical or clinical immunotherapy studies in autoimmune disease. There are protocols with optimized multimer staining methods to detect peptide (p)MHCII+ CD4+ T cells, and some qualified and validated protocols for pMHCI+ CD8+ T cell...

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Detalles Bibliográficos
Autores principales: Patel, Swati, Ramnoruth, Nishta, Wehr, Pascale, Rossjohn, Jamie, Reid, Hugh H, Campbell, Kim, Nel, Hendrik J, Thomas, Ranjeny
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8802177/
https://www.ncbi.nlm.nih.gov/pubmed/35020859
http://dx.doi.org/10.1093/cei/uxab008

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8802177/
https://www.ncbi.nlm.nih.gov/pubmed/35020859
http://dx.doi.org/10.1093/cei/uxab008

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