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Systematic Assessment of the Accuracy of Subunit Counting in Biomolecular Complexes Using Automated Single-Molecule Brightness Analysis

[Image: see text] Analysis of single-molecule brightness allows subunit counting of high-order oligomeric biomolecular complexes. Although the theory behind the method has been extensively assessed, systematic analysis of the experimental conditions required to accurately quantify the stoichiometry...

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Detalles Bibliográficos
Autores principales: Danial, John S. H., Quintana, Yuri, Ros, Uris, Shalaby, Raed, Margheritis, Eleonora G., Chumpen Ramirez, Sabrina, Ungermann, Christian, Garcia-Saez, Ana J., Cosentino, Katia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8802318/
https://www.ncbi.nlm.nih.gov/pubmed/35044771
http://dx.doi.org/10.1021/acs.jpclett.1c03835