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Systematic Assessment of the Accuracy of Subunit Counting in Biomolecular Complexes Using Automated Single-Molecule Brightness Analysis
[Image: see text] Analysis of single-molecule brightness allows subunit counting of high-order oligomeric biomolecular complexes. Although the theory behind the method has been extensively assessed, systematic analysis of the experimental conditions required to accurately quantify the stoichiometry...
Autores principales: | Danial, John S. H., Quintana, Yuri, Ros, Uris, Shalaby, Raed, Margheritis, Eleonora G., Chumpen Ramirez, Sabrina, Ungermann, Christian, Garcia-Saez, Ana J., Cosentino, Katia |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8802318/ https://www.ncbi.nlm.nih.gov/pubmed/35044771 http://dx.doi.org/10.1021/acs.jpclett.1c03835 |
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