TdfH selectively binds metal-loaded tetrameric calprotectin for zinc import

To combat nutritional immunity, N. gonorrhoeae has evolved systems to hijack zinc and other metals directly from host metal-binding proteins such as calprotectin (CP). Here, we report the 6.1 Å cryoEM structure of the gonococcal surface receptor TdfH in complex with a zinc-bound CP tetramer. We furt...

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Autores principales: Bera, Aloke K., Wu, Runrun, Harrison, Simone, Cornelissen, Cynthia Nau, Chazin, Walter J., Noinaj, Nicholas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8803948/
https://www.ncbi.nlm.nih.gov/pubmed/35102276
http://dx.doi.org/10.1038/s42003-022-03039-y
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author Bera, Aloke K.
Wu, Runrun
Harrison, Simone
Cornelissen, Cynthia Nau
Chazin, Walter J.
Noinaj, Nicholas
author_facet Bera, Aloke K.
Wu, Runrun
Harrison, Simone
Cornelissen, Cynthia Nau
Chazin, Walter J.
Noinaj, Nicholas
author_sort Bera, Aloke K.
collection PubMed
description To combat nutritional immunity, N. gonorrhoeae has evolved systems to hijack zinc and other metals directly from host metal-binding proteins such as calprotectin (CP). Here, we report the 6.1 Å cryoEM structure of the gonococcal surface receptor TdfH in complex with a zinc-bound CP tetramer. We further show that TdfH can also interact with CP in the presence of copper and manganese, but not with cobalt.
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spelling pubmed-88039482022-02-07 TdfH selectively binds metal-loaded tetrameric calprotectin for zinc import Bera, Aloke K. Wu, Runrun Harrison, Simone Cornelissen, Cynthia Nau Chazin, Walter J. Noinaj, Nicholas Commun Biol Article To combat nutritional immunity, N. gonorrhoeae has evolved systems to hijack zinc and other metals directly from host metal-binding proteins such as calprotectin (CP). Here, we report the 6.1 Å cryoEM structure of the gonococcal surface receptor TdfH in complex with a zinc-bound CP tetramer. We further show that TdfH can also interact with CP in the presence of copper and manganese, but not with cobalt. Nature Publishing Group UK 2022-01-31 /pmc/articles/PMC8803948/ /pubmed/35102276 http://dx.doi.org/10.1038/s42003-022-03039-y Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Bera, Aloke K.
Wu, Runrun
Harrison, Simone
Cornelissen, Cynthia Nau
Chazin, Walter J.
Noinaj, Nicholas
TdfH selectively binds metal-loaded tetrameric calprotectin for zinc import
title TdfH selectively binds metal-loaded tetrameric calprotectin for zinc import
title_full TdfH selectively binds metal-loaded tetrameric calprotectin for zinc import
title_fullStr TdfH selectively binds metal-loaded tetrameric calprotectin for zinc import
title_full_unstemmed TdfH selectively binds metal-loaded tetrameric calprotectin for zinc import
title_short TdfH selectively binds metal-loaded tetrameric calprotectin for zinc import
title_sort tdfh selectively binds metal-loaded tetrameric calprotectin for zinc import
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8803948/
https://www.ncbi.nlm.nih.gov/pubmed/35102276
http://dx.doi.org/10.1038/s42003-022-03039-y
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