Anti-polyphenol oxidase properties of total flavonoids from young loquat fruits: inhibitory activity and mechanism

This study investigated anti-polyphenol oxidase activity and mechanism of purified total flavonoids (PTF) from young loquat fruits. PTF remarkably inhibited the activity of polyphenol oxidase (PPO) with an IC(50) value of 21.03 ± 2.37 μg/mL. Based on enzyme kinetics, PTF was found to be a potent, mi...

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Detalles Bibliográficos
Autores principales: Huang, Gui-Li, Sun, Ling-Xiang, Ma, Jia-Jia, Sui, Si-Yao, Wang, Yu-Ning
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2021
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8806263/
https://www.ncbi.nlm.nih.gov/pubmed/33587004
http://dx.doi.org/10.1080/21655979.2021.1886387
Descripción
Sumario:This study investigated anti-polyphenol oxidase activity and mechanism of purified total flavonoids (PTF) from young loquat fruits. PTF remarkably inhibited the activity of polyphenol oxidase (PPO) with an IC(50) value of 21.03 ± 2.37 μg/mL. Based on enzyme kinetics, PTF was found to be a potent, mixed-type, and reversible inhibitor of PPO. The fluorescence intensity of PPO was quenched by PTF through forming a PTF-PPO complex in a static procedure. Therefore, this study authenticated PTF as an efficient PPO inhibitor, which would contribute to their utilization in food industry.

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