Anti-polyphenol oxidase properties of total flavonoids from young loquat fruits: inhibitory activity and mechanism

This study investigated anti-polyphenol oxidase activity and mechanism of purified total flavonoids (PTF) from young loquat fruits. PTF remarkably inhibited the activity of polyphenol oxidase (PPO) with an IC(50) value of 21.03 ± 2.37 μg/mL. Based on enzyme kinetics, PTF was found to be a potent, mi...

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Detalles Bibliográficos
Autores principales: Huang, Gui-Li, Sun, Ling-Xiang, Ma, Jia-Jia, Sui, Si-Yao, Wang, Yu-Ning
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2021
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8806263/
https://www.ncbi.nlm.nih.gov/pubmed/33587004
http://dx.doi.org/10.1080/21655979.2021.1886387
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author Huang, Gui-Li
Sun, Ling-Xiang
Ma, Jia-Jia
Sui, Si-Yao
Wang, Yu-Ning
author_facet Huang, Gui-Li
Sun, Ling-Xiang
Ma, Jia-Jia
Sui, Si-Yao
Wang, Yu-Ning
author_sort Huang, Gui-Li
collection PubMed
description This study investigated anti-polyphenol oxidase activity and mechanism of purified total flavonoids (PTF) from young loquat fruits. PTF remarkably inhibited the activity of polyphenol oxidase (PPO) with an IC(50) value of 21.03 ± 2.37 μg/mL. Based on enzyme kinetics, PTF was found to be a potent, mixed-type, and reversible inhibitor of PPO. The fluorescence intensity of PPO was quenched by PTF through forming a PTF-PPO complex in a static procedure. Therefore, this study authenticated PTF as an efficient PPO inhibitor, which would contribute to their utilization in food industry.
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spelling pubmed-88062632022-02-02 Anti-polyphenol oxidase properties of total flavonoids from young loquat fruits: inhibitory activity and mechanism Huang, Gui-Li Sun, Ling-Xiang Ma, Jia-Jia Sui, Si-Yao Wang, Yu-Ning Bioengineered Research Paper This study investigated anti-polyphenol oxidase activity and mechanism of purified total flavonoids (PTF) from young loquat fruits. PTF remarkably inhibited the activity of polyphenol oxidase (PPO) with an IC(50) value of 21.03 ± 2.37 μg/mL. Based on enzyme kinetics, PTF was found to be a potent, mixed-type, and reversible inhibitor of PPO. The fluorescence intensity of PPO was quenched by PTF through forming a PTF-PPO complex in a static procedure. Therefore, this study authenticated PTF as an efficient PPO inhibitor, which would contribute to their utilization in food industry. Taylor & Francis 2021-02-15 /pmc/articles/PMC8806263/ /pubmed/33587004 http://dx.doi.org/10.1080/21655979.2021.1886387 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Huang, Gui-Li
Sun, Ling-Xiang
Ma, Jia-Jia
Sui, Si-Yao
Wang, Yu-Ning
Anti-polyphenol oxidase properties of total flavonoids from young loquat fruits: inhibitory activity and mechanism
title Anti-polyphenol oxidase properties of total flavonoids from young loquat fruits: inhibitory activity and mechanism
title_full Anti-polyphenol oxidase properties of total flavonoids from young loquat fruits: inhibitory activity and mechanism
title_fullStr Anti-polyphenol oxidase properties of total flavonoids from young loquat fruits: inhibitory activity and mechanism
title_full_unstemmed Anti-polyphenol oxidase properties of total flavonoids from young loquat fruits: inhibitory activity and mechanism
title_short Anti-polyphenol oxidase properties of total flavonoids from young loquat fruits: inhibitory activity and mechanism
title_sort anti-polyphenol oxidase properties of total flavonoids from young loquat fruits: inhibitory activity and mechanism
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8806263/
https://www.ncbi.nlm.nih.gov/pubmed/33587004
http://dx.doi.org/10.1080/21655979.2021.1886387
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