Cardiac Gene Therapy With Relaxin Receptor 1 Overexpression Protects Against Acute Myocardial Infarction

Relaxin is a pleiotropic hormone shown to confer cardioprotection in several preclinical models of cardiac ischemia-reperfusion injury. In the present study, the effects of up-regulating relaxin family peptide receptor 1 (RXFP1) via adeno-associated virus serotype 9 (AAV9) vectors were investigated...

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Autores principales: Devarakonda, Teja, Mauro, Adolfo G., Cain, Chad, Das, Anindita, Salloum, Fadi N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Preclinical Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8807852/
https://www.ncbi.nlm.nih.gov/pubmed/35128209
http://dx.doi.org/10.1016/j.jacbts.2021.10.012
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author Devarakonda, Teja
Mauro, Adolfo G.
Cain, Chad
Das, Anindita
Salloum, Fadi N.
author_facet Devarakonda, Teja
Mauro, Adolfo G.
Cain, Chad
Das, Anindita
Salloum, Fadi N.
author_sort Devarakonda, Teja
collection PubMed
description Relaxin is a pleiotropic hormone shown to confer cardioprotection in several preclinical models of cardiac ischemia-reperfusion injury. In the present study, the effects of up-regulating relaxin family peptide receptor 1 (RXFP1) via adeno-associated virus serotype 9 (AAV9) vectors were investigated in a mouse model of myocardial infarction. AAV9-RXFP1 vectors were generated and injected in adult male CD1 mice. Up-regulation of Rxfp1 was confirmed via quantitative polymerase chain reaction, and overexpressing animals showed increased sensitivity to relaxin-induced ventricular inotropic response. Overexpressing animals also demonstrated reduced infarct size and preserved cardiac function 24 hours after ischemia-reperfusion. Up-regulation of RXFP1 via AAV9 vectors has potential therapeutic utility in preventing adverse remodeling after myocardial infarction.
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spelling pubmed-88078522022-02-04 Cardiac Gene Therapy With Relaxin Receptor 1 Overexpression Protects Against Acute Myocardial Infarction Devarakonda, Teja Mauro, Adolfo G. Cain, Chad Das, Anindita Salloum, Fadi N. JACC Basic Transl Sci Preclinical Research Relaxin is a pleiotropic hormone shown to confer cardioprotection in several preclinical models of cardiac ischemia-reperfusion injury. In the present study, the effects of up-regulating relaxin family peptide receptor 1 (RXFP1) via adeno-associated virus serotype 9 (AAV9) vectors were investigated in a mouse model of myocardial infarction. AAV9-RXFP1 vectors were generated and injected in adult male CD1 mice. Up-regulation of Rxfp1 was confirmed via quantitative polymerase chain reaction, and overexpressing animals showed increased sensitivity to relaxin-induced ventricular inotropic response. Overexpressing animals also demonstrated reduced infarct size and preserved cardiac function 24 hours after ischemia-reperfusion. Up-regulation of RXFP1 via AAV9 vectors has potential therapeutic utility in preventing adverse remodeling after myocardial infarction. Elsevier 2021-12-22 /pmc/articles/PMC8807852/ /pubmed/35128209 http://dx.doi.org/10.1016/j.jacbts.2021.10.012 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Preclinical Research
Devarakonda, Teja
Mauro, Adolfo G.
Cain, Chad
Das, Anindita
Salloum, Fadi N.
Cardiac Gene Therapy With Relaxin Receptor 1 Overexpression Protects Against Acute Myocardial Infarction
title Cardiac Gene Therapy With Relaxin Receptor 1 Overexpression Protects Against Acute Myocardial Infarction
title_full Cardiac Gene Therapy With Relaxin Receptor 1 Overexpression Protects Against Acute Myocardial Infarction
title_fullStr Cardiac Gene Therapy With Relaxin Receptor 1 Overexpression Protects Against Acute Myocardial Infarction
title_full_unstemmed Cardiac Gene Therapy With Relaxin Receptor 1 Overexpression Protects Against Acute Myocardial Infarction
title_short Cardiac Gene Therapy With Relaxin Receptor 1 Overexpression Protects Against Acute Myocardial Infarction
title_sort cardiac gene therapy with relaxin receptor 1 overexpression protects against acute myocardial infarction
topic Preclinical Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8807852/
https://www.ncbi.nlm.nih.gov/pubmed/35128209
http://dx.doi.org/10.1016/j.jacbts.2021.10.012
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