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Recruitment of Scc2/4 to double-strand breaks depends on γH2A and DNA end resection
Homologous recombination enables cells to overcome the threat of DNA double-strand breaks (DSBs), allowing for repair without the loss of genetic information. Central to the homologous recombination repair process is the de novo loading of cohesin around a DSB by its loader complex Scc2/4. Although...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Life Science Alliance LLC
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8807874/ https://www.ncbi.nlm.nih.gov/pubmed/35086935 http://dx.doi.org/10.26508/lsa.202101244 |
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author | Scherzer, Martin Giordano, Fosco Ferran, Maria Solé Ström, Lena |
author_facet | Scherzer, Martin Giordano, Fosco Ferran, Maria Solé Ström, Lena |
author_sort | Scherzer, Martin |
collection | PubMed |
description | Homologous recombination enables cells to overcome the threat of DNA double-strand breaks (DSBs), allowing for repair without the loss of genetic information. Central to the homologous recombination repair process is the de novo loading of cohesin around a DSB by its loader complex Scc2/4. Although cohesin’s DSB accumulation has been explored in numerous studies, the prerequisites for Scc2/4 recruitment during the repair process are still elusive. To address this question, we combine chromatin immunoprecipitation-qPCR with a site-specific DSB in vivo, in Saccharomyces cerevisiae. We find that Scc2 DSB recruitment relies on γH2A and Tel1, but as opposed to cohesin, not on Mec1. We further show that the binding of Scc2, which emanates from the break site, depends on and coincides with DNA end resection. Absence of chromatin remodeling at the DSB affects Scc2 binding and DNA end resection to a comparable degree, further indicating the latter to be a major driver for Scc2 recruitment. Our results shed light on the intricate DSB repair cascade leading to the recruitment of Scc2/4 and subsequent loading of cohesin. |
format | Online Article Text |
id | pubmed-8807874 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Life Science Alliance LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-88078742022-02-15 Recruitment of Scc2/4 to double-strand breaks depends on γH2A and DNA end resection Scherzer, Martin Giordano, Fosco Ferran, Maria Solé Ström, Lena Life Sci Alliance Research Articles Homologous recombination enables cells to overcome the threat of DNA double-strand breaks (DSBs), allowing for repair without the loss of genetic information. Central to the homologous recombination repair process is the de novo loading of cohesin around a DSB by its loader complex Scc2/4. Although cohesin’s DSB accumulation has been explored in numerous studies, the prerequisites for Scc2/4 recruitment during the repair process are still elusive. To address this question, we combine chromatin immunoprecipitation-qPCR with a site-specific DSB in vivo, in Saccharomyces cerevisiae. We find that Scc2 DSB recruitment relies on γH2A and Tel1, but as opposed to cohesin, not on Mec1. We further show that the binding of Scc2, which emanates from the break site, depends on and coincides with DNA end resection. Absence of chromatin remodeling at the DSB affects Scc2 binding and DNA end resection to a comparable degree, further indicating the latter to be a major driver for Scc2 recruitment. Our results shed light on the intricate DSB repair cascade leading to the recruitment of Scc2/4 and subsequent loading of cohesin. Life Science Alliance LLC 2022-01-27 /pmc/articles/PMC8807874/ /pubmed/35086935 http://dx.doi.org/10.26508/lsa.202101244 Text en © 2022 Scherzer et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Articles Scherzer, Martin Giordano, Fosco Ferran, Maria Solé Ström, Lena Recruitment of Scc2/4 to double-strand breaks depends on γH2A and DNA end resection |
title | Recruitment of Scc2/4 to double-strand breaks depends on γH2A and DNA end resection |
title_full | Recruitment of Scc2/4 to double-strand breaks depends on γH2A and DNA end resection |
title_fullStr | Recruitment of Scc2/4 to double-strand breaks depends on γH2A and DNA end resection |
title_full_unstemmed | Recruitment of Scc2/4 to double-strand breaks depends on γH2A and DNA end resection |
title_short | Recruitment of Scc2/4 to double-strand breaks depends on γH2A and DNA end resection |
title_sort | recruitment of scc2/4 to double-strand breaks depends on γh2a and dna end resection |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8807874/ https://www.ncbi.nlm.nih.gov/pubmed/35086935 http://dx.doi.org/10.26508/lsa.202101244 |
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