Protocol for inducing inflammation and acute myelin degeneration in larval zebrafish

This protocol details the induction of inflammation and acute myelin degeneration in larval zebrafish with a duration of <10 days. We describe the use of this model to screen the effects of candidate compounds on inflammation, followed by RNA isolation, and qPCR-based quantification of gene expre...

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Detalles Bibliográficos
Autores principales: Jaronen, Merja, Wheeler, Michael A., Quintana, Francisco J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8808285/
https://www.ncbi.nlm.nih.gov/pubmed/35128477
http://dx.doi.org/10.1016/j.xpro.2022.101134
Descripción
Sumario:This protocol details the induction of inflammation and acute myelin degeneration in larval zebrafish with a duration of <10 days. We describe the use of this model to screen the effects of candidate compounds on inflammation, followed by RNA isolation, and qPCR-based quantification of gene expression. We then outline the steps for bioinformatic analysis of the mechanisms associated with the compounds. This protocol can be used in combination with drugs and genetic targeting to identify pathways that contribute to neurodegeneration. For complete details on the use and execution of this profile, please refer to Wheeler et al. (2019).

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