Protocol for measuring weak cellular traction forces using well-controlled ultra-soft polyacrylamide gels

Traction force microscopy (TFM) is a popular technique for studying cellular stresses; however, the reproducible fabrication of ultrasoft substrates for the reliable detection of weak cellular stresses (below 100 Pa) remains a challenge. Here, we describe a simple in vitro TFM protocol using such ul...

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Detalles Bibliográficos
Autores principales: Mustapha, Farah, Sengupta, Kheya, Puech, Pierre-Henri
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8808286/
https://www.ncbi.nlm.nih.gov/pubmed/35128476
http://dx.doi.org/10.1016/j.xpro.2022.101133

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8808286/
https://www.ncbi.nlm.nih.gov/pubmed/35128476
http://dx.doi.org/10.1016/j.xpro.2022.101133

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