Analysis of RNA-containing compartments by hybridization and proximity labeling in cultured human cells

This protocol describes a hybridization-proximity labeling (HyPro) approach for identification of proteins and RNAs co-localizing with a transcript of interest in genetically unperturbed cells. It outlines steps required for purification of a recombinant HyPro enzyme, hybridization of fixed and perm...

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Detalles Bibliográficos
Autores principales: Yap, Karen, Chung, Tek Hong, Makeyev, Eugene V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8808287/
https://www.ncbi.nlm.nih.gov/pubmed/35128480
http://dx.doi.org/10.1016/j.xpro.2022.101139

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8808287/
https://www.ncbi.nlm.nih.gov/pubmed/35128480
http://dx.doi.org/10.1016/j.xpro.2022.101139

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