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Analysis of RNA-containing compartments by hybridization and proximity labeling in cultured human cells

This protocol describes a hybridization-proximity labeling (HyPro) approach for identification of proteins and RNAs co-localizing with a transcript of interest in genetically unperturbed cells. It outlines steps required for purification of a recombinant HyPro enzyme, hybridization of fixed and perm...

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Detalles Bibliográficos
Autores principales:	Yap, Karen, Chung, Tek Hong, Makeyev, Eugene V.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8808287/ https://www.ncbi.nlm.nih.gov/pubmed/35128480 http://dx.doi.org/10.1016/j.xpro.2022.101139

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