Comprehensive interrogation of the ADAR2 deaminase domain for engineering enhanced RNA editing activity and specificity

Adenosine deaminases acting on RNA (ADARs) can be repurposed to enable programmable RNA editing, however their exogenous delivery leads to transcriptome-wide off-targeting, and additionally, enzymatic activity on certain RNA motifs, especially those flanked by a 5’ guanosine is very low thus limitin...

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Detalles Bibliográficos
Autores principales: Katrekar, Dhruva, Xiang, Yichen, Palmer, Nathan, Saha, Anushka, Meluzzi, Dario, Mali, Prashant
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Biochemistry and Chemical Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8809894/
https://www.ncbi.nlm.nih.gov/pubmed/35044296
http://dx.doi.org/10.7554/eLife.75555

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8809894/
https://www.ncbi.nlm.nih.gov/pubmed/35044296
http://dx.doi.org/10.7554/eLife.75555

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