Preparation of screening assays for ADP-ribosyl readers and erasers using the GAP-tag as a binding probe

Here, we describe a protocol to set up a screening assay for ADP-ribosyl binding proteins including proteins that possess O-glycosidase or N-glycosidase activities. The FRET-based assay measures the interaction of any ADP-ribosyl binding protein fused to CFP with a cysteine-ADP-ribosylated GAP-tag f...

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Detalles Bibliográficos
Autores principales: Sowa, Sven T., Galera-Prat, Albert, Wazir, Sarah, Alanen, Heli I., Maksimainen, Mirko M., Lehtiö, Lari
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8810561/
https://www.ncbi.nlm.nih.gov/pubmed/35141567
http://dx.doi.org/10.1016/j.xpro.2022.101147

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8810561/
https://www.ncbi.nlm.nih.gov/pubmed/35141567
http://dx.doi.org/10.1016/j.xpro.2022.101147

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