CRISPR-Cas9 induces large structural variants at on-target and off-target sites in vivo that segregate across generations

CRISPR-Cas9 genome editing has potential to cure diseases without current treatments, but therapies must be safe. Here we show that CRISPR-Cas9 editing can introduce unintended mutations in vivo, which are passed on to the next generation. By editing fertilized zebrafish eggs using four guide RNAs s...

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Detalles Bibliográficos
Autores principales: Höijer, Ida, Emmanouilidou, Anastasia, Östlund, Rebecka, van Schendel, Robin, Bozorgpana, Selma, Tijsterman, Marcel, Feuk, Lars, Gyllensten, Ulf, den Hoed, Marcel, Ameur, Adam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8810904/
https://www.ncbi.nlm.nih.gov/pubmed/35110541
http://dx.doi.org/10.1038/s41467-022-28244-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8810904/
https://www.ncbi.nlm.nih.gov/pubmed/35110541
http://dx.doi.org/10.1038/s41467-022-28244-5

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