CRISPR-Cas9 induces large structural variants at on-target and off-target sites in vivo that segregate across generations

CRISPR-Cas9 genome editing has potential to cure diseases without current treatments, but therapies must be safe. Here we show that CRISPR-Cas9 editing can introduce unintended mutations in vivo, which are passed on to the next generation. By editing fertilized zebrafish eggs using four guide RNAs s...

Descripción completa

Detalles Bibliográficos
Autores principales: Höijer, Ida, Emmanouilidou, Anastasia, Östlund, Rebecka, van Schendel, Robin, Bozorgpana, Selma, Tijsterman, Marcel, Feuk, Lars, Gyllensten, Ulf, den Hoed, Marcel, Ameur, Adam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8810904/
https://www.ncbi.nlm.nih.gov/pubmed/35110541
http://dx.doi.org/10.1038/s41467-022-28244-5

Ejemplares similares

Ejemplares similares