A pan-CRISPR analysis of mammalian cell specificity identifies ultra-compact sgRNA subsets for genome-scale experiments

A genetic knockout can be lethal to one human cell type while increasing growth rate in another. This context specificity confounds genetic analysis and prevents reproducible genome engineering. Genome-wide CRISPR compendia across most common human cell lines offer the largest opportunity to underst...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhao, Boyang, Rao, Yiyun, Leighow, Scott, O’Brien, Edward P., Gilbert, Luke, Pritchard, Justin R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8810922/
https://www.ncbi.nlm.nih.gov/pubmed/35110534
http://dx.doi.org/10.1038/s41467-022-28045-w

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8810922/
https://www.ncbi.nlm.nih.gov/pubmed/35110534
http://dx.doi.org/10.1038/s41467-022-28045-w

Ejemplares similares