CRISPRi enables fast growth followed by stable aerobic pyruvate formation in Escherichia coli without auxotrophy

CRISPR interference (CRISPRi) was applied to enable the aerobic production of pyruvate in Escherichia coli MG1655 under glucose excess conditions by targeting the promoter regions of aceE or pdhR. Knockdown strains were cultivated in aerobic shaking flasks and the influence of inducer concentration...

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Detalles Bibliográficos
Autores principales: Ziegler, Martin, Hägele, Lorena, Gäbele, Teresa, Takors, Ralf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8811725/
https://www.ncbi.nlm.nih.gov/pubmed/35140555
http://dx.doi.org/10.1002/elsc.202100021

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8811725/
https://www.ncbi.nlm.nih.gov/pubmed/35140555
http://dx.doi.org/10.1002/elsc.202100021

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