Highly reliable creation of floxed alleles by electroporating single-cell embryos

BACKGROUND: Floxed (flanked by loxP) alleles are a crucial portion of conditional knockout mouse models. However, an efficient and reliable strategy to flox genomic regions of any desired size is still lacking. RESULTS: Here, we demonstrate that the method combining electroporation of fertilized egg...

Descripción completa

Detalles Bibliográficos
Autores principales: Sentmanat, Monica F., White, J. Michael, Kouranova, Evguenia, Cui, Xiaoxia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8815186/
https://www.ncbi.nlm.nih.gov/pubmed/35115009
http://dx.doi.org/10.1186/s12915-021-01223-w

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8815186/
https://www.ncbi.nlm.nih.gov/pubmed/35115009
http://dx.doi.org/10.1186/s12915-021-01223-w

Ejemplares similares