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Highly reliable creation of floxed alleles by electroporating single-cell embryos

BACKGROUND: Floxed (flanked by loxP) alleles are a crucial portion of conditional knockout mouse models. However, an efficient and reliable strategy to flox genomic regions of any desired size is still lacking. RESULTS: Here, we demonstrate that the method combining electroporation of fertilized egg...

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Detalles Bibliográficos
Autores principales:	Sentmanat, Monica F., White, J. Michael, Kouranova, Evguenia, Cui, Xiaoxia
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2022
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8815186/ https://www.ncbi.nlm.nih.gov/pubmed/35115009 http://dx.doi.org/10.1186/s12915-021-01223-w

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