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Effect of dietary protein levels on the growth, enzyme activity, and immunological status of Culter mongolicus fingerlings

A 65-day growth trial was conducted to investigate the dietary protein requirements for Culter mongolicus fingerlings. Isolipidic and isoenergetic diets were formulated with five dietary protein levels (32%, 37%, 42%, 47%, and 52%). Each diet was assigned to triplicate groups of 70 C. mongolicus fin...

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Autores principales: Qian, Jing, Xiao, Lingjun, Feng, Kai, Li, Wei, Liao, Chuansong, Zhang, Tanglin, Liu, Jiashou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8815975/
https://www.ncbi.nlm.nih.gov/pubmed/35120192
http://dx.doi.org/10.1371/journal.pone.0263507
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author Qian, Jing
Xiao, Lingjun
Feng, Kai
Li, Wei
Liao, Chuansong
Zhang, Tanglin
Liu, Jiashou
author_facet Qian, Jing
Xiao, Lingjun
Feng, Kai
Li, Wei
Liao, Chuansong
Zhang, Tanglin
Liu, Jiashou
author_sort Qian, Jing
collection PubMed
description A 65-day growth trial was conducted to investigate the dietary protein requirements for Culter mongolicus fingerlings. Isolipidic and isoenergetic diets were formulated with five dietary protein levels (32%, 37%, 42%, 47%, and 52%). Each diet was assigned to triplicate groups of 70 C. mongolicus fingerlings (0.99±0.08 g). The results indicated that weight gain and specific growth rate (SGR) increased with increasing dietary protein levels up to 47%. The activities of intestinal trypsin and lipase were the lowest in the 32% protein and 52% protein groups, while amylase activity reduced markedly in the 47% protein group. These results suggest that different dietary protein levels may cause different transformations of nutrients. The activities of superoxide dismutase (SOD) and lysozyme were not affected by varying dietary protein levels, except for those in the 32% protein group. In contrast, the content of malondialdehyde (MDA) increased with increasing dietary protein levels and reaching a maximum in the 52% protein group, suggesting that MDA accumulation depends on the protein concentration and the potential oxidative stress. Taken together, based on the broken-line analysis of SGR, we recommended the optimum dietary protein for C. mongolicus fingerlings to be 48.97%~49.31%.
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spelling pubmed-88159752022-02-05 Effect of dietary protein levels on the growth, enzyme activity, and immunological status of Culter mongolicus fingerlings Qian, Jing Xiao, Lingjun Feng, Kai Li, Wei Liao, Chuansong Zhang, Tanglin Liu, Jiashou PLoS One Research Article A 65-day growth trial was conducted to investigate the dietary protein requirements for Culter mongolicus fingerlings. Isolipidic and isoenergetic diets were formulated with five dietary protein levels (32%, 37%, 42%, 47%, and 52%). Each diet was assigned to triplicate groups of 70 C. mongolicus fingerlings (0.99±0.08 g). The results indicated that weight gain and specific growth rate (SGR) increased with increasing dietary protein levels up to 47%. The activities of intestinal trypsin and lipase were the lowest in the 32% protein and 52% protein groups, while amylase activity reduced markedly in the 47% protein group. These results suggest that different dietary protein levels may cause different transformations of nutrients. The activities of superoxide dismutase (SOD) and lysozyme were not affected by varying dietary protein levels, except for those in the 32% protein group. In contrast, the content of malondialdehyde (MDA) increased with increasing dietary protein levels and reaching a maximum in the 52% protein group, suggesting that MDA accumulation depends on the protein concentration and the potential oxidative stress. Taken together, based on the broken-line analysis of SGR, we recommended the optimum dietary protein for C. mongolicus fingerlings to be 48.97%~49.31%. Public Library of Science 2022-02-04 /pmc/articles/PMC8815975/ /pubmed/35120192 http://dx.doi.org/10.1371/journal.pone.0263507 Text en © 2022 Qian et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Qian, Jing
Xiao, Lingjun
Feng, Kai
Li, Wei
Liao, Chuansong
Zhang, Tanglin
Liu, Jiashou
Effect of dietary protein levels on the growth, enzyme activity, and immunological status of Culter mongolicus fingerlings
title Effect of dietary protein levels on the growth, enzyme activity, and immunological status of Culter mongolicus fingerlings
title_full Effect of dietary protein levels on the growth, enzyme activity, and immunological status of Culter mongolicus fingerlings
title_fullStr Effect of dietary protein levels on the growth, enzyme activity, and immunological status of Culter mongolicus fingerlings
title_full_unstemmed Effect of dietary protein levels on the growth, enzyme activity, and immunological status of Culter mongolicus fingerlings
title_short Effect of dietary protein levels on the growth, enzyme activity, and immunological status of Culter mongolicus fingerlings
title_sort effect of dietary protein levels on the growth, enzyme activity, and immunological status of culter mongolicus fingerlings
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8815975/
https://www.ncbi.nlm.nih.gov/pubmed/35120192
http://dx.doi.org/10.1371/journal.pone.0263507
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