Escherichia coli recombinant expression of SARS-CoV-2 protein fragments

We have developed a method for the inexpensive, high-level expression of antigenic protein fragments of SARS-CoV-2 proteins in Escherichia coli. Our approach uses the thermophilic family 9 carbohydrate-binding module (CBM9) as an N-terminal carrier protein and affinity tag. The CBM9 module was joine...

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Detalles Bibliográficos
Autores principales: McGuire, Bailey E., Mela, Julia E., Thompson, Vanessa C., Cucksey, Logan R., Stevens, Claire E., McWhinnie, Ralph L., Winkler, Dirk F. H., Pelech, Steven, Nano, Francis E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8817660/
https://www.ncbi.nlm.nih.gov/pubmed/35123472
http://dx.doi.org/10.1186/s12934-022-01753-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8817660/
https://www.ncbi.nlm.nih.gov/pubmed/35123472
http://dx.doi.org/10.1186/s12934-022-01753-0

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