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Escherichia coli recombinant expression of SARS-CoV-2 protein fragments
We have developed a method for the inexpensive, high-level expression of antigenic protein fragments of SARS-CoV-2 proteins in Escherichia coli. Our approach uses the thermophilic family 9 carbohydrate-binding module (CBM9) as an N-terminal carrier protein and affinity tag. The CBM9 module was joine...
Autores principales: | McGuire, Bailey E., Mela, Julia E., Thompson, Vanessa C., Cucksey, Logan R., Stevens, Claire E., McWhinnie, Ralph L., Winkler, Dirk F. H., Pelech, Steven, Nano, Francis E. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8817660/ https://www.ncbi.nlm.nih.gov/pubmed/35123472 http://dx.doi.org/10.1186/s12934-022-01753-0 |
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