Protocol for generation and regeneration of PEG-passivated slides for single-molecule measurements

Single-molecule fluorescence detection by total internal reflection microscope requires surface passivation by polyethylene glycol (PEG) coating, which is labor intensive and is only good for one or two experiments. Here, we present an efficient and reliable protocol for generating and regenerating...

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Detalles Bibliográficos
Autores principales: Paul, Tapas, Myong, Sua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8819390/
https://www.ncbi.nlm.nih.gov/pubmed/35146451
http://dx.doi.org/10.1016/j.xpro.2022.101152

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8819390/
https://www.ncbi.nlm.nih.gov/pubmed/35146451
http://dx.doi.org/10.1016/j.xpro.2022.101152

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