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Protocol for generation and regeneration of PEG-passivated slides for single-molecule measurements
Single-molecule fluorescence detection by total internal reflection microscope requires surface passivation by polyethylene glycol (PEG) coating, which is labor intensive and is only good for one or two experiments. Here, we present an efficient and reliable protocol for generating and regenerating...
Autores principales: | Paul, Tapas, Myong, Sua |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8819390/ https://www.ncbi.nlm.nih.gov/pubmed/35146451 http://dx.doi.org/10.1016/j.xpro.2022.101152 |
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