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Laser microirradiation as a tool to investigate the role of liquid-liquid phase separation in DNA damage repair
Here we describe a protocol for the generation of site-specific DNA damage, including double and single strand breaks, using the 405 nm laser of a confocal microscope in cells pre-sensitized with Hoechst. This is a simple approach, particularly useful to assess the involvement of proteins and the ro...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8819395/ https://www.ncbi.nlm.nih.gov/pubmed/35146448 http://dx.doi.org/10.1016/j.xpro.2022.101146 |