Cargando…

Enhanced Transgene Expression by Optimization of Poly A in Transfected CHO Cells

The generation of the stable, high-level recombinant protein-producing cell lines remains a significant challenge in the biopharmaceutical industry. Expression vector optimization is an effective strategy to increase transgene expression levels and stability, and the choice of suitable poly A elemen...

Descripción completa

Detalles Bibliográficos
Autores principales:	Wang, Xiao-yin, Du, Qiu-jie, Zhang, Wei-li, Xu, Dan-hua, Zhang, Xi, Jia, Yan-long, Wang, Tian-yun
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2022
Materias:	Bioengineering and Biotechnology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8819543/ https://www.ncbi.nlm.nih.gov/pubmed/35141210 http://dx.doi.org/10.3389/fbioe.2022.722722

Ejemplares similares

Stabilizing and Anti-Repressor Elements Effectively Increases Transgene Expression in Transfected CHO Cells
por: Li, Qin, et al.
Publicado: (2022)

The Effect of microRNA on the Production of Recombinant Protein in CHO Cells and its Mechanism
por: Liu, Hui-Ning, et al.
Publicado: (2022)

Enhancing and stabilizing monoclonal antibody production by Chinese hamster ovary (CHO) cells with optimized perfusion culture strategies
por: Liang, Kexue, et al.
Publicado: (2023)

Two human MARs effectively increase transgene expression in transfected CHO cells
por: Li, Qin, et al.
Publicado: (2018)

Human rhinovirus internal ribosome entry site element enhances transgene expression in transfected CHO-S cells
por: Chai, Yu-rong, et al.
Publicado: (2018)

Decoupling Growth and Protein Production in CHO Cells: A Targeted Approach
por: Donaldson, James S., et al.
Publicado: (2021)

Sonoporation-Mediated Gene Transfection: A Novel Direction for Cell Reprogramming In Vivo
por: Du, Meng, et al.
Publicado: (2022)

Engineering protein glycosylation in CHO cells to be highly similar to murine host cells
por: Gupta, Shivani, et al.
Publicado: (2023)

Extracellular Nanovesicle Enhanced Gene Transfection Using Polyethyleneimine in HEK293T Cells and Zebrafish Embryos
por: Zhang, Zhenzhen, et al.
Publicado: (2020)

An Overview of Methods and Tools for Transfection of Eukaryotic Cells in vitro
por: Fus-Kujawa, Agnieszka, et al.
Publicado: (2021)

Functional Trimeric SARS-CoV-2 Envelope Protein Expressed in Stable CHO Cells
por: Mayrhofer, Patrick, et al.
Publicado: (2021)

Mechanistic insights into the biological activity of S-Sulfocysteine in CHO cells using a multi-omics approach
por: Nguyen, Melanie, et al.
Publicado: (2023)

Pranoprofen Nanoparticles With Poly(L-Lactide)-b-Poly(Ethylene Glycol)-b-Poly(L-Lactide) as the Matrix Toward Improving Ocular Anti-inflammation
por: Luo, Yang, et al.
Publicado: (2020)

Model-based intensification of CHO cell cultures: One-step strategy from fed-batch to perfusion
por: Richelle, Anne, et al.
Publicado: (2022)

Selection of biophysically favorable antibody variants using a modified Flp-In CHO mammalian display platform
por: Huhtinen, Olli, et al.
Publicado: (2023)

Infectious viruses from transfected SARS-CoV-2 genomic RNA
por: Väisänen, Elina, et al.
Publicado: (2023)

Dynamic Modeling of CHO Cell Metabolism Using the Hybrid Cybernetic Approach With a Novel Elementary Mode Analysis Strategy
por: Martínez, Juan A., et al.
Publicado: (2020)

Growth and eGFP Production of CHO-K1 Suspension Cells Cultivated From Single Cell to Laboratory Scale
por: Schmitz, Julian, et al.
Publicado: (2021)

Brain Derived Neurotrophic Factor and Glial Cell Line-Derived Neurotrophic Factor-Transfected Bone Mesenchymal Stem Cells for the Repair of Periphery Nerve Injury
por: Zhang, Qiang, et al.
Publicado: (2020)

A CHO-Based Cell-Free Dual Fluorescence Reporter System for the Straightforward Assessment of Amber Suppression and scFv Functionality
por: Krebs, Simon K., et al.
Publicado: (2022)

Enhancing stability of recombinant CHO cells by CRISPR/Cas9-mediated site-specific integration into regions with distinct histone modifications
por: Hertel, Oliver, et al.
Publicado: (2022)

Hybrid deep modeling of a CHO-K1 fed-batch process: combining first-principles with deep neural networks
por: Pinto, José, et al.
Publicado: (2023)

Enhanced ε-Poly-L-Lysine Production by the Synergistic Effect of ε-Poly-L-Lysine Synthetase Overexpression and Citrate in Streptomyces albulus
por: Wang, Aixia, et al.
Publicado: (2020)

The EF‐1α promoter maintains high‐level transgene expression from episomal vectors in transfected CHO‐K1 cells
por: Wang, Xiaoyin, et al.
Publicado: (2017)

Encapsulation of Andrographolide in poly(lactide-co-glycolide) Nanoparticles: Formulation Optimization and in vitro Efficacy Studies
por: Oseni, Bukola A., et al.
Publicado: (2021)

Optimization for the efficient recovery of poly(3-hydroxybutyrate) using the green solvent 1,3-dioxolane
por: Wongmoon, Chanakarn, et al.
Publicado: (2022)

Customized Barrier Membrane (Titanium Alloy, Poly Ether-Ether Ketone and Unsintered Hydroxyapatite/Poly-l-Lactide) for Guided Bone Regeneration
por: Shi, Yilin, et al.
Publicado: (2022)

Preparation and optimization of poly (lactic-co-glycolic acid) rod-shaped particles in nano size range for paclitaxel delivery
por: Xu, Mengyao, et al.
Publicado: (2022)

Epsilon-poly-L-lysine: Recent Advances in Biomanufacturing and Applications
por: Wang, Liang, et al.
Publicado: (2021)

Efficacy Evaluation of Ciprofloxacin-Loaded Poly (Trimethylene Carbonate) Implants in the Treatment of Chronic Osteomyelitis
por: Liu, Yixiu, et al.
Publicado: (2022)

Proteomic Profiling of IgG1 Producing CHO Cells Using LC/LC-SPS-MS(3): The Effects of Bioprocessing Conditions on Productivity and Product Quality
por: Strasser, Lisa, et al.
Publicado: (2021)

Advances in the development and optimization strategies of the hemostatic biomaterials
por: Guo, Yayuan, et al.
Publicado: (2023)

Transcriptomic Analysis of Mature Transgenic Poplar Expressing the Transcription Factor JERF36 Gene in Two Different Environments
por: Zhang, Weixi, et al.
Publicado: (2022)

Construction and Optimization of the de novo Biosynthesis Pathway of Mogrol in Saccharomyces Cerevisiae
por: Wang, Siyu, et al.
Publicado: (2022)

An Integrative Dual-Layer Poly-L-Lactic Acid Fibrous Membrane Prevents Peritendinous Adhesions
por: Wang, Wei, et al.
Publicado: (2020)

Biodegradable membrane of poly(l-lactide acid-dioxanone-glycolide) and stereocomplex poly(lactide) with enhanced crystallization and biocompatibility
por: Fan, Tiantang, et al.
Publicado: (2022)

Catalytic Features and Thermal Adaptation Mechanisms of a Deep Sea Bacterial Cutinase-Type Poly(Ethylene Terephthalate) Hydrolase
por: Liu, Yu, et al.
Publicado: (2022)

Time Optimal Trajectory Planing Based on Improved Sparrow Search Algorithm
por: Zhang, Xiaofeng, et al.
Publicado: (2022)

Identification of a potent MAR element from the human genome and assessment of its activity in stably transfected CHO cells
por: Tian, Zheng‐Wei, et al.
Publicado: (2017)

Optimizing a Fed-Batch High-Density Fermentation Process for Medium Chain-Length Poly(3-Hydroxyalkanoates) in Escherichia coli
por: Scheel, Ryan A., et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_3m9tedmt1cjt9m6aqrn2u924jh