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Single-cell isoform analysis in human immune cells

High-throughput single-cell analysis today is facilitated by protocols like the 10X Genomics platform or Drop-Seq which generate cDNA pools in which the origin of a transcript is encoded at its 5′ or 3′ end. Here, we used R2C2 to sequence and demultiplex 12 million full-length cDNA molecules generat...

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Detalles Bibliográficos
Autores principales: Volden, Roger, Vollmers, Christopher
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8819920/
https://www.ncbi.nlm.nih.gov/pubmed/35130954
http://dx.doi.org/10.1186/s13059-022-02615-z