Analysis of N(6)-Methyladenosine Methylome in Adenocarcinoma of Esophagogastric Junction

Background: From previous studies, we found that there are more than 100 types of RNA modifications in RNA molecules. m(6)A methylation is the most common. The incidence rate of adenocarcinoma of the esophagogastric junction (AEG) at home and abroad has increased faster than that of stomach cancer a...

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Autores principales: Huang, Jia-Bin, Hu, Bin-Bin, He, Rong, He, Lian, Zou, Chen, Man, Chang-Feng, Fan, Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8820482/
https://www.ncbi.nlm.nih.gov/pubmed/35140740
http://dx.doi.org/10.3389/fgene.2021.787800
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author Huang, Jia-Bin
Hu, Bin-Bin
He, Rong
He, Lian
Zou, Chen
Man, Chang-Feng
Fan, Yu
author_facet Huang, Jia-Bin
Hu, Bin-Bin
He, Rong
He, Lian
Zou, Chen
Man, Chang-Feng
Fan, Yu
author_sort Huang, Jia-Bin
collection PubMed
description Background: From previous studies, we found that there are more than 100 types of RNA modifications in RNA molecules. m(6)A methylation is the most common. The incidence rate of adenocarcinoma of the esophagogastric junction (AEG) at home and abroad has increased faster than that of stomach cancer at other sites in recent years. Here, we systematically analyze the modification pattern of m(6)A mRNA in adenocarcinoma at the esophagogastric junction. Methods: m(6)A sequencing, RNA sequencing, and bioinformatics analysis were used to describe the m(6)A modification pattern in adenocarcinoma and normal tissues at the esophagogastric junction. Results: In AEG samples, a total of 4,775 new m(6)A peaks appeared, and 3,054 peaks disappeared. The unique m6A-related genes in AEG are related to cancer-related pathways. There are hypermethylated or hypomethylated m(6)A peaks in AEG in differentially expressed mRNA transcripts. Conclusion: This study preliminarily constructed the first m(6)A full transcriptome map of human AEG. This has a guiding role in revealing the mechanism of m(6)A-mediated gene expression regulation.
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spelling pubmed-88204822022-02-08 Analysis of N(6)-Methyladenosine Methylome in Adenocarcinoma of Esophagogastric Junction Huang, Jia-Bin Hu, Bin-Bin He, Rong He, Lian Zou, Chen Man, Chang-Feng Fan, Yu Front Genet Genetics Background: From previous studies, we found that there are more than 100 types of RNA modifications in RNA molecules. m(6)A methylation is the most common. The incidence rate of adenocarcinoma of the esophagogastric junction (AEG) at home and abroad has increased faster than that of stomach cancer at other sites in recent years. Here, we systematically analyze the modification pattern of m(6)A mRNA in adenocarcinoma at the esophagogastric junction. Methods: m(6)A sequencing, RNA sequencing, and bioinformatics analysis were used to describe the m(6)A modification pattern in adenocarcinoma and normal tissues at the esophagogastric junction. Results: In AEG samples, a total of 4,775 new m(6)A peaks appeared, and 3,054 peaks disappeared. The unique m6A-related genes in AEG are related to cancer-related pathways. There are hypermethylated or hypomethylated m(6)A peaks in AEG in differentially expressed mRNA transcripts. Conclusion: This study preliminarily constructed the first m(6)A full transcriptome map of human AEG. This has a guiding role in revealing the mechanism of m(6)A-mediated gene expression regulation. Frontiers Media S.A. 2022-01-24 /pmc/articles/PMC8820482/ /pubmed/35140740 http://dx.doi.org/10.3389/fgene.2021.787800 Text en Copyright © 2022 Huang, Hu, He, He, Zou, Man and Fan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Huang, Jia-Bin
Hu, Bin-Bin
He, Rong
He, Lian
Zou, Chen
Man, Chang-Feng
Fan, Yu
Analysis of N(6)-Methyladenosine Methylome in Adenocarcinoma of Esophagogastric Junction
title Analysis of N(6)-Methyladenosine Methylome in Adenocarcinoma of Esophagogastric Junction
title_full Analysis of N(6)-Methyladenosine Methylome in Adenocarcinoma of Esophagogastric Junction
title_fullStr Analysis of N(6)-Methyladenosine Methylome in Adenocarcinoma of Esophagogastric Junction
title_full_unstemmed Analysis of N(6)-Methyladenosine Methylome in Adenocarcinoma of Esophagogastric Junction
title_short Analysis of N(6)-Methyladenosine Methylome in Adenocarcinoma of Esophagogastric Junction
title_sort analysis of n(6)-methyladenosine methylome in adenocarcinoma of esophagogastric junction
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8820482/
https://www.ncbi.nlm.nih.gov/pubmed/35140740
http://dx.doi.org/10.3389/fgene.2021.787800
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