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Optimization of Ribosome Footprinting Conditions for Ribo-Seq in Human and Drosophila melanogaster Tissue Culture Cells

Our understanding of mRNA translation and its regulation has been transformed by the development of ribosome profiling. This approach relies upon RNase footprinting of translating ribosomes in a precise manner to generate an accurate snapshot of ribosome positions with nucleotide resolution. Here we...

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Detalles Bibliográficos
Autores principales: Douka, Katerina, Agapiou, Michaela, Birds, Isabel, Aspden, Julie L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8822167/
https://www.ncbi.nlm.nih.gov/pubmed/35145996
http://dx.doi.org/10.3389/fmolb.2021.791455