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4090 Dysregulation of Skeletal Muscle Mitochondrial Function following Critical Illness: a Translational Approach
OBJECTIVES/GOALS: The objective of the study was to determine whether CLP altered genes associated with mitochondrial function in the diaphragm. METHODS/STUDY POPULATION: A rodent cecal-ligation and puncture (CLP) model used to mimic sepsis-induced critical illness. The CLP model involved ligation o...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cambridge University Press
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8823613/ http://dx.doi.org/10.1017/cts.2020.296 |
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author | Gill, Luther Simon, Liz Molina, Patricia |
author_facet | Gill, Luther Simon, Liz Molina, Patricia |
author_sort | Gill, Luther |
collection | PubMed |
description | OBJECTIVES/GOALS: The objective of the study was to determine whether CLP altered genes associated with mitochondrial function in the diaphragm. METHODS/STUDY POPULATION: A rodent cecal-ligation and puncture (CLP) model used to mimic sepsis-induced critical illness. The CLP model involved ligation of 50% of the cecum below the ileocecal valve in adult C57BL6 mice, followed by needle puncture of the cecum resulting in mid-grade sepsis. Mice survived for 48 hours or more, following injury. Diaphragm and limb muscles were harvested 24 hours following CLP (N = 6) and following a sham CLP procedure (N = 6). RESULTS/ANTICIPATED RESULTS: Gene expression of mitochondrial related genes (mef2c, myh1, pgc1-α), were significantly decreased in the diaphragm of CLP injured animals when compared to controls. In addition, ubiquitin ligases, genes associated with skeletal muscle atrophy murf1 and atrogin were increased in the diaphragm 24 hours after injury (p< 0.01). DISCUSSION/SIGNIFICANCE OF IMPACT: Our results indicate that sepsis-induced critical illness significantly impacts the expression of genes implicated in mitochondrial homeostasis and atrophy. Ongoing studies will identify whether CLP injury decreases skeletal muscle mitochondrial function. |
format | Online Article Text |
id | pubmed-8823613 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Cambridge University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-88236132022-02-18 4090 Dysregulation of Skeletal Muscle Mitochondrial Function following Critical Illness: a Translational Approach Gill, Luther Simon, Liz Molina, Patricia J Clin Transl Sci Mechanistic Basic to Clinical OBJECTIVES/GOALS: The objective of the study was to determine whether CLP altered genes associated with mitochondrial function in the diaphragm. METHODS/STUDY POPULATION: A rodent cecal-ligation and puncture (CLP) model used to mimic sepsis-induced critical illness. The CLP model involved ligation of 50% of the cecum below the ileocecal valve in adult C57BL6 mice, followed by needle puncture of the cecum resulting in mid-grade sepsis. Mice survived for 48 hours or more, following injury. Diaphragm and limb muscles were harvested 24 hours following CLP (N = 6) and following a sham CLP procedure (N = 6). RESULTS/ANTICIPATED RESULTS: Gene expression of mitochondrial related genes (mef2c, myh1, pgc1-α), were significantly decreased in the diaphragm of CLP injured animals when compared to controls. In addition, ubiquitin ligases, genes associated with skeletal muscle atrophy murf1 and atrogin were increased in the diaphragm 24 hours after injury (p< 0.01). DISCUSSION/SIGNIFICANCE OF IMPACT: Our results indicate that sepsis-induced critical illness significantly impacts the expression of genes implicated in mitochondrial homeostasis and atrophy. Ongoing studies will identify whether CLP injury decreases skeletal muscle mitochondrial function. Cambridge University Press 2020-07-29 /pmc/articles/PMC8823613/ http://dx.doi.org/10.1017/cts.2020.296 Text en © The Association for Clinical and Translational Science 2020 https://creativecommons.org/licenses/by/4.0/This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Mechanistic Basic to Clinical Gill, Luther Simon, Liz Molina, Patricia 4090 Dysregulation of Skeletal Muscle Mitochondrial Function following Critical Illness: a Translational Approach |
title | 4090 Dysregulation of Skeletal Muscle Mitochondrial Function following Critical Illness: a Translational Approach |
title_full | 4090 Dysregulation of Skeletal Muscle Mitochondrial Function following Critical Illness: a Translational Approach |
title_fullStr | 4090 Dysregulation of Skeletal Muscle Mitochondrial Function following Critical Illness: a Translational Approach |
title_full_unstemmed | 4090 Dysregulation of Skeletal Muscle Mitochondrial Function following Critical Illness: a Translational Approach |
title_short | 4090 Dysregulation of Skeletal Muscle Mitochondrial Function following Critical Illness: a Translational Approach |
title_sort | 4090 dysregulation of skeletal muscle mitochondrial function following critical illness: a translational approach |
topic | Mechanistic Basic to Clinical |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8823613/ http://dx.doi.org/10.1017/cts.2020.296 |
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