Cargando…

In-Cell NMR of Intact Mammalian Cells Preserved with the Cryoprotectants DMSO and Glycerol Have Similar DNP Performance

NMR has the resolution and specificity to determine atomic-level protein structures of isotopically-labeled proteins in complex environments and, with the sensitivity gains conferred by dynamic nuclear polarization (DNP), NMR has the sensitivity to detect proteins at their endogenous concentrations....

Descripción completa

Detalles Bibliográficos
Autores principales: Xiao, Yiling, Ghosh, Rupam, Frederick, Kendra K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8824258/
https://www.ncbi.nlm.nih.gov/pubmed/35145995
http://dx.doi.org/10.3389/fmolb.2021.789478